Abstract:

Objective To investigate the expression of miR⁃128⁃3p in colorectal cancer（CRC）and its effect on the invasion and migration of CRC cells and the underlying mechanism. Methods The canceroustissues and paired para⁃cancerous tissues of CRC patients（n = 56）who underwent surgical treatment in our hospital from⁃ June 2015 to May 2017 were collected. CRC cell lines and human normal colorectal mucosal cells were obtained. The expression of miR⁃128⁃3p in CRC tissues and cells were detected by qPCR and its relationship with the clinico⁃ pathologic feature of CRC patient was further analyzed. miR⁃128⁃3p mimic，mimic NC，miR⁃128⁃3p inhibitor and inhibitor NC were transfected into SW620 cells，respectively. Transwell assay was used to detected the invasion and migration of SW620 cells. Luciferase reporter assay was used to validate the targeted relationship between miR⁃128⁃3p and ZEB1. After transfection with ZEB1plasmid alone or in combination with miR⁃128⁃3p mimics，the invasion and migration ability of SW620 cells was detected by transwell assay，the expression of ZEB1 protein or mRNA in SW620 cells was detected by WB and qPCR，respectively. Results miR⁃128⁃3p was down⁃regulated in CRC cells and tissues（P < 0.05），and its expression level was related to the degree of tumor differentiation， lymph node metastasis and distant metastasis（P < 0.05）. Up⁃regulated expression of miR⁃128⁃3pcould significantly inhibit the invasion and migration of SW620 cells and down⁃regulated expression of miR⁃128⁃3p reversed the inhibi⁃ tory effect of miR⁃128⁃3p mimics on SW620 cells. Theluciferase reporter assay confirmed that miR⁃128⁃3p directly targeted to regulated ZEB1. Over ⁃ expression of ZEB1 could significantly promote the invasion and migration of SW620 cells，whereas the collaborative transfection of miR⁃128⁃3p mimics could down⁃regulate the expression of ZEB1 protein and mRNA，and reduce the invasion and migration ability of SW620 cells. Conclusions The low expression of miR⁃128⁃3pwas correlated with tumor differentiation，lymph node metastasis and distant metastasis of CRC patients. miR⁃128⁃3p could inhibit the invasion and migration of CRC cells by targeting ZEB1

Key words:

colorectal cancer, miR?128?3p, ZEB1, invasion, migration